1 Federal University of Agriculture, Makurdi

2 University of Port Harcourt, Choba



This present study was aimed at investigating the acute toxicity of organotin on fresh water shrimps and its resistance to marine bacteria. 200 water shrimps were exposed to varying concentrations of Tributyltin Chloride (TBTCL) and Diphenyltin Chloride (DPTCL)   for 96 hours and a probit was used to determine the lethal dose (LD50). 200g of sediment from Onne sea port Rivers State was manually polluted by TBTCL and DPTCL for 56 days. Organotin resistant bacteria were screened on mineral salt medium at different concentration of the organotin using the spread plate technique. Results from this study showed a lethal doze (LD50) of 4.24mg/l after 24 hours and 1.97mg/l after 48 hours for TBTCl on fresh water shrimps and a lethal doze of 21.05mg/l after 24 hours, 0.83mg/l after 48 hours and 0.006mg/l after 72 hours for DPTCl. The total viable count of bacteria obtained from varying concentrations of TBTCl   indicates that approximately 65% of bacterial populations were resistant to 3.0mM of TBTCl and DPTCI since these isolates could grow on MSA supplemented with TBTCI and DPTCI. Statistically, there was no significant difference between the bacterial loads between the different concentrations of TBTCL. Acute toxicity effect of TBTCl and DPTCl on fresh water shrimps reveals TBTCl and DPTCl as one of the toxic substances in the marine ecosystem however; marine bacteria can be harnessed for their resistant abilities.